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Erdal Toprak , Assistant Professor of Pharmacology and in the Cecil H. and Ida Green Comprehensive Center for Molecular, Computational, and Systems Biology and a Southwestern Medical Foundation Scholar in Biomedical Research at UT Southwestern. To attack this problem, two UTSW labs with different focuses came together to seek a solution. Dr. Greenberg had been trying to create new antibiotics using PPMOs also called anti-sense molecules because they can mimic DNA or RNA and bind to mRNAs genetic sequence like the other half of a zipper, blocking the bacterial machinery for building proteins. Meanwhile, Dr. Topraks lab was using a device he had developed to study how bacteria become resistant to antibiotics. About a year ago, his research team identified a mutation in E. coli that increased drug sensitivity by blocking an efflux pump complex called AcrAB-TolC. The two scientists and their labs then joined forces to create a PPMO that could target this important E. coli complex.
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